|Number of Members||9|
|Highest Ranking Member||Lightning, 2nd place|
|Lowest Ranking Member||Staci, 14th place|
In Bigger! Badder! Brutal-er!, the Toxic Rats were formed by which contestant arrived at the finish line after escaping the wildlife. Lightning and Jo arrived first, and Lightning was placed on the Toxic Rats. Scott arrived third, and became the second member. B arrived fifth after Brick and became the third member. Dawn arrived eighth after Zoey and Mike, but still became the fourth member. Dakota and Sam arrived 10th and 11th and became the 5th and 6th members, followed closely by Staci who became the last member. Before learning their team name, Lightning suggested it as "Team Lightning" or "Lightning Squad," but Chris revealed that the teams and the names were chosen by himself. The Toxic Rats performed well in the first challenge, being able to beat the Mutant Maggots even after a late start in the log race. However, their cabin was destroyed in the process. Chris declared the Maggots as the winners. Due to Staci's constant annoying chatter, she was voted off first in the Toxic Rat's first marshmallow ceremony. However, since they had a total of seven players, this elimination made both teams even.
In Truth or Laser Shark, the Toxic Rats are seen in their cabins. Dawn and Dakota are on the girls side and Lightning, Scott, B, and Sam are on the boys side. Scott went looking for the Chris Head, but was unable to find it. At the challenge, it is revealed that Sam farted on his first and only date and that B's real name is Beverly. The two teams tie in the first part of the challenge. In Part 2, the Toxic Rats evidently lose the second part, sending them to the campfire ceremony. Before the elimination, Scott and Dawn tell their team that keeping Dakota would be a bad idea. Sam wants to keep her, due to his attraction towards her. However, Dakota, who lost the challenge for the Toxic Rats, was voted out. Sam was seen disappointed.
In Ice Ice Baby, the team wins the first part of the challenge because of B's brilliant idea. Scott does not like this and wants B out as soon as possible. In the second part, the teams must throw snowballs, while trying to steal the other team's flag. Scott makes himself captain and chooses Sam and Lightning to steal the flag. In reality, Scott plans to throw the challenge. B makes a magnifying glass to help the team; however, Scott messes with it and makes the team lose. Scott blames the loss on B. Before the campfire ceremony, Scott convinces his team that B needs to go. The marshmallows go to Dawn, Lightning, and Scott. Before the eliminated contestant is announced, Dakota flies into the ceremony on a glider. She asks for more screen-time. Chris makes her an intern. The Marshmallow of Toxic Loserdom goes to B. Right before he gets hurled, he attempts to say something. However, Chris launches him into the sky before he could speak.
In Backstabbers Ahoy!, the Rats lost again and Dawn was eliminated from the game due to being framed by Scott as he claimed that she stole all the contestants' items when in reality it was him. Dawn tries to prove Scott is evil, but fails as she was put in a bag and was launched out.
In Runaway Model, the Toxic Rats lost another challenge and Sam got the boot due to being useless by the others. That same episode, Chris switches Scott from the Rats to the Mutant Maggots and Jo from the Maggots to the Toxic Rats.
- B (eliminated)
- Dakota (eliminated)
- Dawn (eliminated)
- Sam (eliminated)
- Scott (switched to the Mutant Maggots in episode six)
- Staci (eliminated)
- Brick - After his team lost the challenge, Brick offered to be switched on to the opposing team in episode four.
- Jo - After Sam's elimination, Chris told to the Mutant Maggots and the Toxic Rats to choose two people to swap teams. Then Jo and Scott swap teams due to Scott wanting to manipulate Zoey.
|Name||Sex||Rank voted off on Team||Rank in game||Episode voted off||Reason voted off|
|Staci||Female||8th||14th||Bigger! Badder! Brutal-er!||She annoyed her other teammates due to her compulsive chatter and lies.|
|Dakota||Female||7th||13th||Truth or Laser Shark||Scott convinced Sam, Lightning, Dawn and B to vote her off, as she was with the paparazzi and made her team lose as they were supposed to win.|
|B||Male||6th||12th||Ice Ice Baby||Scott convinced Sam, Lightning, and Dawn to vote him off.|
|Dawn||Female||5th||11th||Backstabbers Ahoy!||Dawn found out that Scott purposely lost the water skiing challenge, eventually Scott found out that she knows. Then, he was able to get his team to vote her off by framing Dawn by stealing an object the contestants likes the most and throwing them in a plastic bag, saying that Dawn stole them.|
|Sam||Male||4th||10th||Runaway Model||Chef took his video games, causing him to see everything as a digital videogame, causing him to lose the challenge for his team.|
|Brick||Male||3rd||9th||A Mine is a Terrible Thing to Waste||He helped his former team, costing his team the win.|
|Jo||Female||2nd||5th||Up Up and Away||She was eliminated because of her attitude towards the others and was viewed as a threat.|
|Lightning||Male||1st||2nd||Brain vs. Brawn: The Ultimate Showdown||He lost to Cameron in the final two challenge, after he was attacked by all the radioactive monsters from the competition.|
- The Toxic Rats has the most members in Total Drama: Revenge of the Island.
- On September 27, 2011, Christian Potenza confirmed that the name for the Radioactive Rats was scrapped, and on December 13, 2011, it was revealed that the new name for the team was "The Toxic Rats."
- They are the first team in Total Drama: Revenge of the Island to lose a challenge and eliminate a member from their team.
- This team seems to have a conflict with one of its own members (Staci).
- As of Backstabbers Ahoy!, every female member of the Toxic Rats have been eliminated.
- However, Jo is moved on the team in episode six.
- Staci and Sam are the only members of this team to not be eliminated because of Scott, so far.